Yes, but not just with the minimal tools available on Ancestry.

You can go to gedmatch.com, sign up for a free account and then upload your Ancestry raw data there. Do the same with your mother and sister. Use the phasing utility to create artificial kits for your mother's and father's genomes. You can then get lists of your paternal and maternal matches.

You can also install a special extension called Ancestry DNA Helper on a chrome browser. That enables you to scan the match lists for any kits you manage and make lists of those who share common matches--for example, you and your mother. Those who don't match your mother are more likely to be on your paternal side.

You may also find it useful to review this guide before starting:

https://sites.google.com/site/wheatonsurname/beginners-guide...

Jim