I also descend directly from this line. I think that Anna/Hannah Hesser d Mar 1833 in Fayette Co, OH and is buried at the Coffman Orr Cemetery. She was living with daughter Elizabeth who m. Henry Snider. He is buried there, too. Elizabeth remarried, but I'm not sure to whom. The Anna Hesser buried there was b 1737. Would make her our girl.
As to the will in Loudoun Co, VA, have you read it? Could it be the daughter of Conrad and Anna, who was also named Anna? I don't know whom she married.