Daniel Isham married  Margaret Howell at Hungar's Parish, Northampton Co. VA., Susannah Thomas ca. 1661, Northampton Co. VA.,  Gertrude Bowman Cropper after 1668 Northampton Co. Va. He had one child with Margaret, who died between 1660 and 1661. The other children were by Susannah Thomas. There were no children with Gertrude Bowan Cropper who was the widow of John Cropper I.