Your Welcome! Have found more information on them, they evidently moved back to Missouri in the 1860's.

1870 Missouri, Putnam Co, Richland Twnship, Unionville
family 77-77
Applegate, Jacob, 43, Farmer, 1000-450, MO
Elizabeth, 39, Keeping house, KY
Melvina, 14, daughter, KY
Annie, 11, daughter, KY
Henry, 10,son KY
Katie, 7, daughter,KY
Mollie, 4, KY

1880 Missouri, Putnam-Union Dist#209, Unionville
Applegate, Jacob, 53, Farmer, MO, KY, KY *note ill chronic diarrhea
Elizabeth, keeps house, KY, PA, KY
Mary P.,14, daughter KY, MO, KY